Elution with a reverse gradient is also demonstrated at low salt concentrations for these proteins. Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity. GE Life Sciences 17-5451-01 Capto Phenyl High Sub 25mL Analytical Reagents Industrial & Scientific Lab & Scientific Products Lab Chemicals Analytical. One of the mAbs and lysozyme exhibit U-shaped retention factor curves and for each, we determine the critical gradient slope beyond which 100% recovery is no longer possible. Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity.

Experimental results are presented for two different monoclonal antibodies and for lysozyme on Capto Phenyl (High Sub) resin. We show that the normalized gradient slope determines whether the protein elutes in the gradient, partially elutes, or is trapped in the column. Two dynamic models, one based on the local equilibrium theory and the other based on the linear driving force approximation, are presented. Capto Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein. For these conditions, a steep gradient can overtake the protein in the column, causing it to rebind. During gradient elution the salt concentration is gradually decreased from a high value thereby reducing the retention factor and increasing the protein chromatographic velocity. In some cases, however, protein retention also increases at low salt concentrations resulting in a U-shaped retention factor curve. In general, an increase in salt concentration drives protein partitioning to the hydrophobic surface while a decrease reduces it. Protein retention in hydrophobic interaction chromatography is described by the solvophobic theory as a function of the kosmostropic salt concentration. Low yield is seen for: Capto Butyl, Phenyl Sepharose 6 Fast Flow (high sub), and Capto Phenyl (high sub) indicating that the hydrophobic interaction of.

Capto phenyl high sub